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10.1177/1934578x1200700626 134 NiJ.ChenH.ZhangC.LuoQ.QinY.YangY.et al (2022)

Approved advanced therapies now include anti-tumor necrosis factor alpha (anti-TNF-) agents, anti-integrins, cytokine inhibitors targeting IL-12/23 and IL-23, Janus kinase (JAK) inhibitors, and sphingosine-1-phosphate receptor (S1PR) modulators

To verify that the SAT1 disruption cassettes were successfully excised from GDH2/gdh2 and GDH3/gdh3 strains, genomic PCR was used with primers C1 and C4 to amplify the region between the upper end of 5 flank sequences to the lower end of 3 flank sequences to make sure that both alleles of each gene were removed (Supplementary Fig