doi: 10.1016/j.immuni.2021.12.013
The primary antibodies included anti-progerin (1:50, Santa Cruz, sc-81611), anti-Lamin A/C (1:2000, CST, 4777), anti-p53 (1:1000, Abcam, ab131442), anti-p21 (1:1000, Abcam, ab109199), anti-PI3K (1:1000, CST, 4249), anti-p-AKT1 (S473) (1:1000, Proteintech, 66444-1-Ig), anti-AKT1 (1:1000, CST, 2938), and anti-GAPDH (1:1000, Proteintech, 60004-1)
At the same time, the tubing demonstrates excellent compatibility with phenol-containing formulations by maintaining low extractables and resisting chemical degradation during prolonged exposure
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