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The following have been previously described: vps29 20 , snx1 51 , snx2a 51 , snx2b 51 , vps9a 57 , mon1-2 45 , ccz1a 58 , ccz1b 58 , vps35a-1b-2c-1 7 , vps35b-1c-1 7 , GFP-VSR1 59 , VPS29-GFP 20 , SNX1-GFP/Ch 8 , mRFP-SYP32 (WAVE22) 60 , Ch-RAB5 (WAVE7) 60 , Ch-RAB7 (WAVE5) 60 , VHA-a1-mRFP 61 , mCIT-PH OSBP (P7Y) 62
Rising levels of NAPQI in the liver cause widespread damage, including lipid peroxidation, inactivation of cellular proteins, and disruption of DNA metabolism (Bessems 2001)
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Termini were capped, glu187 was set to the partially protonated state based on protonation predictions within Maestro, and the systems were embedded in POPC: POPE = 3:1 membrane bilayers with equal numbers of lipids on both leaflets