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Elderly people may also have vitamin B12 absorption problems, as will people with celiac disease or Crohns disease

The most common GLP-1 side effects are gastrointestinal (nausea, constipation, diarrhea) and usually ease over weeks

It is a statutory requirement under Section 1860D-2(e)(2)(A) of the Social Security Act, added by the Medicare Prescription Drug, Improvement, and Modernization Act of 2003

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Add 1,000 L (1 mL) of reconstitution solvent to the 1 mg vial This yields a 1 mg/mL (1,000 g/mL) stock solution To achieve 100 g/mL working stock: Dilute 100 L of 1 mg/mL stock into 900 L of PBS or cell culture medium yields 100 g/mL in 1 mL To achieve 10 ng/mL in a 5 mL experiment: Take 0.5 L of 100 g/mL working stock + 4,999.5 L of medium Alternatively: dilute to an intermediate concentration to reduce pipetting error Use a dilution series approach (stock working stock final experimental concentration) to minimize pipetting inaccuracy at nanogram-level concentrations