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As such, its important to understand the guidelines for reconstitution when preparing peptides

Add one bead volume of Blocking/Elution Buffer, mix well by pipetting several times, and incubate at room temperature for 5-10 minutes to elute the bound biotinylated molecule from the magnetic beads

12S13S21

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This shows that eliminating the C28-C31 disulfides ability to receive electrons does not alter the observed kinetics of the reaction, thus defining the last reoxidation phase observed in the U597C net single turnover reaction to be associated with electron distribution through the C596-U597 C-terminus and not the interaction with the C28-C31 disulfide