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Exploration of geneexpression modulation : GHKCu has been reported to up or downregulate large panels of genes associated with tissue remodeling, oxidative stress control, and repair processes in various cell types

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Isolation of nuclear and cytoplasmic proteins Nuclear and cytoplasmic proteins were isolated using the NE-PER Nuclear and Cytoplasmic Extraction Reagents kit following the detailed instructions provided by the manufacturer (Thermo scientific, Rockford, IL, USA)