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These alterations in protein expression could potentially be ascribed to the intricate regulatory network of the NRF2/KEAP1 complex: Under homeostatic conditions, NRF2 is continuously ubiquitinated and targeted for 26S proteasomal degradation by its negative regulator KEAP1
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Samples in extraction mix were vortexed for 10 min at 4 C and centrifuged at 15,000 rpm for 10 min at 4 C to pellet insoluble material
Glutathione binds to these toxins through a process called conjugation, which makes them water soluble, allowing the liver and kidneys to eliminate them
Values are mean SEM
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