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By contrast, for HIV, the addition of complement to in vitro assays has been associated with increased antibody-dependent enhancement (ADE) 111 (Box 1) and enhanced HIV transmission 112

Immunoblot assays Tissue samples were crushed on dry ice to obtain homogenous aliquots and then lysed in RIPA buffer (Thermo Scientific #89900) containing Halt protease & phosphatase inhibitor (Thermo Scientific #1861280)

Sun Y, Campisi J, Higano C, Beer TM, Porter P, Coleman I et al

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