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The endogenous GSH could trigger the release of NO and active Pt (II) from SPNA simultaneously (Figures 3E, F), thereby generating highly toxic peroxynitrite anion (ONOO )
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The EEOs were incubated overnight at 4 C with primary antibodies: GLP-1R (MAB7F38, DSHB 1:30) and Ki-67 (sc-23900, Santa Cruz, 1:100), diluted in washing buffer (0.1% Triton X-100 and 0.2% BSA in 1 PBS)
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