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At about 24 h post-transfection, cells were lysed in Pierce IP Lysis Buffer (Thermo Fisher Scientific) supplemented with Halt protease inhibitor cocktail (Thermo Fisher Scientific) for 30 min on ice

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Unlike non-selective GHRPs that activate multiple hormone axes, Ipamorelin provides clean data on GHS-R1a signaling

For guidance on the broader landscape of peptides used in metabolic and hormonal health, see Peptides for Insulin Resistance: GLP-1, Tesamorelin & Emerging Compounds