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Prior to the ITC experiments, concentrated ShD14, ShHTL1, ShHTL4, and ShHTL7 were exchanged into a buffer consisting of 20 mM HEPES (pH 8.0) and 150 mM NaCl to remove dithiothreitol and were then adjusted to a final concentration of 150 M
In recent years, studies have shown liraglutide and other GLP-1 agonists can greatly reduce intracranial pressure in the brain a speculative trigger for migraines
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