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Mechanical shear force from shaking can denature a peptide's secondary structure and permanently reduce its bioactivity

In foods, and fortified B12 occurs naturally in animal-based foods such as meat, fish, eggs, and dairy

Once the water is in, remove the syringe

Either 0.5 L of 3 mM AsA or 5 mM GSH dissolved in buffer (10 mM Bis-Tris, pH 6.5, 30% (w/v) PEG 3350 and 1 mM DTT) was added to each drop of fully grown crystals and maintained for 72 h before the diffraction test