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Essentially, the gap detection test is a variant of the PPI test, with the main distinction being that PPI occurs in a quiet environment 100 ms before a burst of white noise (50 ms at 110 dB SPL), preceded by a 50 ms pure tone (16 kHz, 70 dB SPL)
The in vitro screening revealed that several compounds, including gallic acid, rosmarinic acid, 3-hydroxytyrosol, trans -resveratrol, EGCG, quercetin, and fisetin, exhibit significant inhibitory activities against both AChE and BChE, suggesting their potential to modulate cholinergic neurotransmission