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A SureScript First-Strand cDNA Synthesis Kit (GeneCopoeia, United States) was used to reverse-transcribe the cDNA

Key mechanisms include: Stimulation of collagen and elastin synthesis in fibroblasts Activation of lysyl oxidase for collagen cross-linking Enhanced production of glycosaminoglycans and decorin Modulation of metalloproteinase activity for controlled matrix breakdown The copper ion serves as an essential cofactor for enzymes involved in collagen synthesis and provides antioxidant protection through superoxide dismutase activation

Met Ions Biol Syst 36:139 Stohs SJ, Bagchi D (1995) Oxidative mechanisms in the toxicity of metal ions

Improve insulin resistance

Injections can be once a month, once a week, or twice a week