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There was a substantial increase in intracellular uptake of semaglutide by gastric epithelial cells with SNAC exposure compared with control, which was not apparent with EDTA, a modulator of tight junction function

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Immunoblots were visualized with horseradish peroxidase-conjugated secondary antibodies and a SuperSignal chemiluminescent detection system (Thermo Scientific), using GAPDH as a loading control

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