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IR flox/flox mice, initially generated from C57BL/6J and CD-1 background, had been backcrossed for at least five generations on a C57BL6 background, and Col2-Cre mice, initially established on a C57BL6/129sv background, had been backcrossed for 10 generations on a C57BL6 background before being intercrossed with IR flox/flox mice
In this method Free and Total GSH are measured in the same well, with only one sample required for both results
With one set of 20 strokes and another set of 10 strokes, the remaining sample was homogenized using a 2-ml homogenizer
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