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Antigen retrieval was performed in 10 mM citrate buffer, pH 6.0 using pressure cooker (at 125 C for 5 min)

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Hence, ASK1 and especially JNK inhibitors are highly effective in preventing APAP-induced mitochondrial dysfunction, oxidant stress, and cell death in murine and human hepatocytes [56,59,60] and in vivo [45,59,61,62]