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Additional information Peer review information Nature Metabolism thanks Dominique Langin and the other, anonymous reviewers for their contribution to the peer review of this work
The assay plate was incubated for 3 h at 37 C in 5% CO 2 and allowed to equilibrate at room temperature for 5 min after which 30 L SteadyLite Plus (PerkinElmer, 6066759) was added to each well
This is a cornerstone of responsible lab practice
For semaglutide specifically, studies found the average duration was about 47 days
An FDA-approved drug is reviewed as a specific product with defined manufacturing controls, labeling, and evidence for approved uses
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