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Cell viability was assessed using the CCK-8 assay (Dojindo, Japan), and optical density (OD) was measured at 450 nm using a VARIOSKAN LUX reader (Thermo Fisher Scientific, USA)
The profound fatigue, unpredictable heart rates, and cognitive fog are not just "in your head"they are the direct result of measurable physiological disruptions, including severe oxidative stress, mitochondrial damage, and systemic glutathione depletion
Cell 68 , 879887 (1992)
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