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Step 2: Clean the vial Wipe the rubber stopper of your GHK-Cu vial with an alcohol swab

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The model was refined against one half map, and FSC curves were calculated between this model and the final cryo-EM map (full dataset, blue) of the outcome of model refinement with a half map versus the same map (orange), and of the outcome of model refinement with a half map versus the other half map (green)