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Literature review
Bacterial titers were determined via plaque assays by serially diluting the bacteria in 12-well plates containing confluent Vero cells
Research indicates that individuals with diabetes often have lower levels of glutathione, which can exacerbate oxidative damage and lead to complications such as neuropathy, retinopathy, and cardiovascular diseases
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The biofilm suspension was then serially diluted in 1 PBS and 100 ml aliquots were spread on TSB plates and incubated for 24 h at 37C
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