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Escherichia coli were harvested by centrifugation and resuspended in HEPES buffer (20 mM HEPES, 300 mM NaCl, pH 7.4)

While body weight and composition can influence drug distribution, the half-life of these specific medications is not significantly altered by body weight

doi: 10.1002/2327-6924.12510

If GI side effects are manageable, add cagrilintide at 0.25 mg weekly starting in week 5, while simultaneously increasing retatrutide to 2 mg as per its standard titration

It is an investigational medicinal product (IMP) and has not received a marketing authorisation from the Medicines and Healthcare products Regulatory Agency (MHRA)