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Nuclear and cytoplasmic extracts were analyzed by western blot

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Cells were then incubated for 4 min at room temperature, centrifuged at 1000 x g for 3 min, washed once with 2 mL fresh HC -Ura-Leu medium and finally resuspended in fresh HC -Ura-Leu medium to an OD 600 of 7.5

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GLP-1 receptors in your brain that were suppressing appetite become less activated, leading to increased hunger hormones like ghrelin