The systems were then heated for 100 ps by incrementing the temperature from 0 to 300 K under a constant pressure of 1 atm and periodic boundary conditions with a timestep of 1 fs
In this regard, it can be noted that transcription of the gdhA gene initiates from two promoters, one weak, constitutively expressed, called gdhA P1, and another, more potent, called gdhA P2, transactivated by GdhR, a transcriptional regulator whose DNA binding is inhibited by -ketoglutarate (Pagliarulo et al., 2004)
These longer cycles require more careful monitoring and may benefit from slight dose reductions in weeks 13-16 to maintain receptor sensitivity
It is safer to replace the vial instead of the danger of contamination
The logistics are manageable, but specifics matter