Between 2 and 1 h before the dark phase, rats were injected with either MTII or vehicle in randomized order using the same procedure as described previously ( POMC and AgRP in Situ Hybridization In a separate study, using the same dietary protocol, rats were subjected again to CHOW ( n = 7), fcHS ( n = 7), fcHF ( n = 7), or fcHFHS ( n = 7) for 4 weeks
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