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If the OFV values reduction was more than 3.84 ( 2 , = 0.05, degrees of freedom (d.f.) = 1), the corresponding covariable met the inclusion criteria

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2.9 Cell culture and treatment Mouse colon epithelial cells (MCECs) were cultured in high-glucose Dulbeccos Modified Eagle Medium, supplemented with 10% fetal bovine serum and 1% penicillin-streptomycin (P/S), in a humidified incubator with 5% CO 2 at 37C