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All animals were kept under controlled temperature (22 1 C) in a 12:12 h light:dark cycle, starting at 7:00 a.m., with food and water ad libitum and relative humidity 55 10%
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The next day, the samples were washed twice in 100% methanol for 30 min, cooled down to 4 C in 1 h and bleached in chilled fresh 5% H 2 O 2 in methanol (one volume 35% H 2 O 2 to six times volume methanol) overnight at 4 C
If the powder appears white or off-white, the copper may be absent or improperly complexed
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