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Mouse Immobilized Stress Protocol and Tissue Correction The 6-week-old male ApoE / mice ( n = 25) were randomly assigned into the following two groups for the first phase of the experiment: The control group (HF-C, n = 12) received a high-fat (HF) diet (21.00% fat and 0.15% cholesterol) only, and the stress group (HF-S, n = 13) received the same HF diet and were subjected to restraint stress (12 weeks) ( ApoE / mice fed a HF diet were divided to another two groups: Stress 2 group (Stress): the mice subjected to restraint stress for 12 weeks as mentioned above, and the stress + pitavastatin group (S-Pis): Except for HF diet and restraint stress (12 weeks), the mice were treated with pitavastatin (1 mg/kg/d)

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1 2 3 4 5 6 7 8 9 10 11 12 13 Lehninger principles of biochemistry (7th ed.)

Inject it into a muscle, preferably in the buttocks

What dosing adjustments should I make if I need to start a second cycle after only 3 weeks washout