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Appetite was assessed during the MTT using validated [20] visual analogue scales (VAS) at frequent intervals, followed by an ad libitum meal test [20]
Repeated washing was done with 1X phosphate saline buffer (PBS) to release out excess stain
Supplementing with topical GHK-Cu can help restore some of these lost protective functions by delivering copper ions directly to the scalp, where they serve as essential cofactors for enzymes involved in tissue repair and hair follicle maintenance
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They should therefore be described as preliminary evidence for a mixed intradermal formulation, not proof that injecting GHK-Cu alone treats androgenetic alopecia
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