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RNA extraction and RTqPCR Total RNA from cells was isolated via TRIzol (Takara, Otsu, Japan) and subsequently purified with chloroform/isopropanol/ethanol, and cDNA was synthesized via the PrimeScript RT reagent Kit (Takara) according to the manufacturers instructions and used as the template for qRTPCR
10.1038/sj.thj.6200407 31 FrazerD
The Metabolic Profile of Down Syndrome Down syndrome is a complex metabolic and genetic disorder whose root cause, trisomy 21, is an extra copy of chromosome 21 [59]
SH-SY5Y cells were seeded in 96-well plates at a density of ~ 5000/ per well, and they were exposed to various concentrations (10, 25, 50, 100, and 200 g/mL) of DNZ-NLPs for 24 h