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The supernatants were transferred into a new tube containing a SpinX HPLC 0.2-m nylon microcentrifuge filter (Costar, Corning, NY, United States), centrifuged again at 10,000 g for 2 min at 4C, and stored at 80C until used
If you have doses remaining as the BUD approaches, use them according to schedule but be extra diligent about storage
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