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After washing with Tris-buffered saline containing 0.05% Tween-20 (TBST), the membrane was incubated with horseradish peroxidase-conjugated secondary antibodies (diluted 1 : 3000) for 1 h at room temperature

This reaction is catalyzed by 3-hydroxy-3-methylglutaryl-CoA synthase 2 (HMGCS2)