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Generally speaking, the longer you go without adequate vitamin B12, the more serious your symptoms are
Cells were blocked with 5% BSA in PBS and incubated with primary antibodies overnight
Air exposure
Both cysteines are conserved in most chloroplastic orthologs from terrestrial plants whereas only Cys119 is present in the cytosolic AtAPK3 or in algal representatives (Figure 2 and Supplementary Table 3)
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