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Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse GROa/CXCL1

The takeaway here is that GHK-Cu is structural and slow

This study was funded by the National Natural Science Foundation of China (82374525), Scientific Research Project of the Education Department of Hunan Province (22A0250), Hunan Provincial Clinical Medical Research Center for Ophthalmic Diseases (Traditional Chinese Medicine) (2023SK4038), Key Scientific Research Project of Hunan Administration of Traditional Chinese Medicine (C2023013), Project on Advancing Clinical Evidence - based Capacity for Traditional Chinese Medicine - treated Diseases with Advantages (JBGS-A1-02) and Graduate Innovation Project of Hunan University of Chinese Medicine (2024CX025)

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