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Peptides were then separated across a 5 101 mm Betasil C8 column (Thermo Fisher Scientific) with a linear gradient of 1240% acetonitrile in 0.3% formic acid over a short five minute gradient to limit back exchange with the solvent
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It could be hypothesized that there is an association between the PK of the various GLP-1 RAs, the duration of receptor activation/occupancy and the extent of the HR effect
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