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Angiogenesis and Microvascular Recruitment In animal studies on hind limb ischemia models, where blood flow is severely restricted, BPC-157 was shown to increase vessel density and blood flow recovery

25 The topical application of growth factors was successful in accelerating healing of full-thickness wound in normal mice

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For Safranin O/Fast Green staining, slides were stained with hematoxylin (Mayer, Sigma) for 5 min, 0.005% Fast Green (Thermo Fisher Scientific, Pittsburgh, PA, United States) for 1 min, rinsed with 1% acetic acid, and stained with 0.1% Safranin O (Millipore, Billerica, MA, United States) for 20 min