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Samples were cleared by centrifugation at 4 C 10,000 rpm for 5 min, and the supernatants were analyzed and quantified with the SAM standard curve
In addition, astrocytes, which normally support neurons, become dysfunctional through a process called astrogliosis, which worsens glutamate imbalance and reduces neuronal support (Boille et al., 2006)
Otherwise, your body is just going to get rid of it
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