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POMC-derived peptides including adrenocorticotropin (ACTH), alpha melanocyte stimulating hormone (-MSH), -MSH and -endorphins, MC1R, adrenergic and cholinergic agents, and growth factors, cytokines, or nitric oxide (NO) secreted in the local microenvironment are all involved in the regulation of melanogenesis [6]
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doi:10.1111/ijd.70133
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Samples were then incubated at room temperature for 24 h with goat anti-chicken Alexa Fluor 488 (1:1000 dilution
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