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Phosphate buffered saline (PBS) (pH 7.4, 10) was obtained from Vivantis (Malaysia)
Proteomic analysis of unbounded cellular compartments: synaptic clefts
The absence of allosteric modulation from the RAMP ECD allows the CTR ECD to adopt a conformation that sits further from the receptor TMD than the position observed in the consensus cryo-EM map
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