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The acylation on K20 P by Glu-2OEG linker and C18 fatty diacid moiety that enables enhanced binding to plasma albumin and extended the peptide half-life in vivo 29 were not resolved in both structures, indicating a high conformational flexibility, in line with the recently published cryo-EM structure of semaglutide-bound GLP-1R 30 and our molecular dynamics (MD) simulation results (Supplementary Fig