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For general educational awareness, the principles that govern reconstitution of lyophilised injectable medicines in regulated clinical settings include: Preparation must take place in an appropriate environment (ideally a pharmacy aseptic unit or clinical clean area) using sterile equipment

Pregnancy and Breastfeeding Use only if recommended by your healthcare professional after evaluating the benefits and risks

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Health Canada has stated that unauthorized injectable peptides cannot lawfully be compounded for human use, and the FDA's December 2024 PCAC review recommended against permitting AOD-9604 in 503A compounding in the United States

In practice, bacteriostatic water is used when multiple injections or withdrawals will be needed (thanks to its preservative), whereas sterile water is used when only one-time immediate use is required