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Acknowledgements I would like to thank my former PhD students and post-docs Anne Raben, Anne Flint, Camilla Verdich, Birgitte Sloth, Helle Harder, Anita Belza and Thomas Meinert Larsen for their invaluable contributions to the research described in this article

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Given the increasing clinical and commercial relevance of Semaglutide, we have developed and validated a liquid chromatographytandem mass spectrometry (LC-MS/MS) method that ensures high precision, accuracy, and reproducibility for Semaglutide quantification

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