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After blocking in 5% skimmed milk in PBS containing 0.05% Tween 20, membranes were probed with anti-human Lyn monoclonal mouse antibody (Santa Cruz) diluted 1:1000, or anti-human IL-1 sRII mouse antibody, Clone 8.5 32 followed by detection with anti-mouse-IgG-horseradish peroxidase conjugate diluted at 1:5000 in blocking buffer (Stratagene)
We can further study the most effective form of Se in the antioxidant system of aquatic animals, selectively prevent the oxidative stress of aquaculture animals, clarify the mechanism of selenoproteins in the antioxidant system of aquatic animals, and provide direction for the study of Se toxicity
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