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Imaging was then conducted using laser scanning confocal microscopy, which provided high-resolution images for detailed analysis, and the results were subsequently quantified using ImageJ software (National Institute of Mental Health, Bethesda, MD, USA)

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Notably, the unusual enrichment of Ovarian steroidogenesis in all-male rats suggests a potential resistance to MAFLD progression, as estrogen inhibits abnormal fat deposition and appears to resist fibrosis