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The sections were added with citric acid buffer, cooked in a microwave oven for 3 min, incubated with antigen retrieval solution at room temperature for 10 min, and washed with PBS three times

Garten A et al

Water-soluble vitamins (the B complex and vitamin C) dissolve in water and enter the bloodstream directly through the small intestine

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Material and methods For this study, 48 adult male NMRI mice were divided into two control and experimental groups