Following the manufacturers instructions, total RNA was extracted from the samples using the TRIzol reagent (Invitrogen, CA, USA)
Am J Physiol Lung Cell Mol Physiol 292 : L824L832 Lee KS, Kim SR, Park HS, Park SJ, Min KH, Lee KY, Choe YH, Hong SH, Han HJ, Lee YR, Kim JS, Atlas D, Lee YC 2007 A novel thiol compound, n-acetylcysteine amide, attenuates allergic airway disease by regulating activation of nf-kappab and hypoxia-inducible factor-1alpha
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Finally, the blood samples were nitrated with nitric acid, and the ruthenium content was determined by ICP-MS