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Most clients who book B12 shots come in on a regular cadence (every one to four weeks) for energy support or as part of a methylation protocol with their physician

Plain sterile water is not preservative-free bacteriostatic once the vial seal is broken, it is susceptible to microbial contamination and should only be used for single-draw applications

Clinical indications include deficiency due to inadequate diet or malabsorption, drug-induced deficiency (for example, from long-term isoniazid or oral contraceptives), and certain inborn errors of metabolism (such as pyridoxine-dependent seizures or sideroblastic anemia)

Sasaki Y et al

Turn it upside down and gently pull the plunger out to draw in the prescribed amount of B12 solution into the barrel