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B12 is water-soluble, so the body clears what it does not use

I've brought this up before on this forum and got all the same kind of advice you got, and I do everything properly, and I've been doing it once or twice a week for years, so for me it's just become a fact of life that it's going to hurt

Specificity of staining was confirmed by staining with a rat IgG (6-001-F, R&D Systems, Bio-Techne), a Rabbit IgG (ab27478, Abcam) or a mouse IgG1, kappa (Abcam, ab18443) isotype controls

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