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When the cells reached approximately 80% confluency, cells were harvested with trypsin and seeded at a density of 2.9 10 5 cells per well in 2.5 ml of culture medium into 6 well flat-bottomed plates (Costar, Corning, USA) and incubated for 24 hr

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Furthermore, when compared to the TMX-treated group, administration of LC + TMX resulted in a significant improvement in ionic electrolyte ( F Na + = 2.701, P = 0.001

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