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At the end of the pre-incubation, the cells were washed and placed into a bicarbonate-buffered Krebs solution containing 120 mM NaCl, 4.8 mM KCl, 2.5 mM CaCl 2 , 1.2 mM MgCl 2 , 24 mM NaHCO 3 , 1 g/l BSA, 10 mM HEPES, and 8 mM glucose